Genetic Similarities Among Iranian Populations of Festuca, Lolium, Bromus and Agropyron Using Amplified Fragments Length Polymorphism (AFLP) Markers

نویسندگان

  • Aghafakhr Mirlohi Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, P.O. Box 84156-8311, I.R. Iran
  • Mohammad Mahdi Majidi Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, P.O. Box 84156-8311, I.R. Iran
چکیده مقاله:

The study of genetic variation and phylogenetic relationships is essential for the efficient selection of superior plant material and conducting introgression breeding programs. In Iran, despite the wide geographical distribution of grasses no report is available on the genetic diversity and relationships of cool season grass populations. In this study amplified fragment length polymorphism (AFLP)  was used to study 42 populations from eight species of Festuca arundinacea  Schereb., Festuca. pratensis Huds., Festuca. rubra L., Festuca. ovina L., Lolium perenne L., L. rigidum Gaud., Bromus tomentellus Boiss. and Agropyron cristatum (L) Gaertn.  The number of amplified products ranged from 11 to 78 per primer combination and a total of 497 markers were scored. Jaccard’s genetic similarity coefficients among populations ranged from 0.15 to 0.88 showing high levels of inter and intra-specific genetic diversity. The cluster analysis and principle coordinate analysis (PCOA) reflected the phylogenetic relationships among species and clearly demonstrated differences in the degree of similarity among accessions. Results indicated that AFLP is a useful technique to reveal genetic diversity at different taxonomic levels of grasses and might facilitate the selective introgression of useful genes in plant breeding programs.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

genetic similarities among iranian populations of festuca, lolium, bromus and agropyron using amplified fragments length polymorphism (aflp) markers

the study of genetic variation and phylogenetic relationships is essential for the efficient selection of superior plant material and conducting introgression breeding programs. in iran, despite the wide geographical distribution of grasses no report is available on the genetic diversity and relationships of cool season grass populations. in this study amplified fragment length polymorphism (af...

متن کامل

Analysis of the Genetic Diversity 12 Iranian Damask Rose (Rosa damascena Mill.) Genotypes Using Amplified Fragment Length Polymorphism Markers

In this study, the genetic diversity of 12 Iranian Damask rose (Rosa damascena Mill.) genotypes was studied using amplified fragment length polymorphism (AFLP) markers. Twelve AFLP primer combinations generated 483 polymorphic bands and showed extreme variability and genetic complexity among the studied genotypes. The AFLP analysis revealed a specific amplified fragment for the genotypes collec...

متن کامل

Assessment of Genetic Diversity in Some Iranian Populations of Bunium persicum Using RAPD and AFLP Markers

The genetic diversity of 20 Iranian populations of Bunium persicum has been evaluated with random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers. Fresh leaves of seedlings from each population were used for genomic DNA extraction. Analysis of banding patterns of 15 RAPD primers and 17 AFLP primer combinations, revealed 192 (86%) and 228 (75%) polymorp...

متن کامل

Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers.

A linkage map of rye, previously developed using DS2 x RXL10 F2 mapping population, was enriched with 179 AFLP and 19 RAPD marker loci. The current map covers 1386 cM and contains 480 markers including 200 RFLPs, 179 AFLPs, 88 RAPDs, 12 protein loci and one dwarfing gene. AFLPs generated by EcoRI/MseI primer combinations were distributed over the entire genome as distinct loci or clusters of 2-...

متن کامل

Identification of Satsuma Mandarin (Citrus unshiu) Cultivars in California Using Amplified Fragment Length Polymorphism (AFLP) Markers

Satsuma mandarin (Citrus unshiu) is one of the most important types of citrus grown world-wide, especially in China and Japan. It is also produced in California from mid-October to January. Several new Satsuma mandarin cultivars, originally from Japan or China, were introduced into California in the past few years. We intend to identify different Satsuma mandarin cultivars in California using A...

متن کامل

Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers.

Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1)...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 8  شماره 1

صفحات  16- 23

تاریخ انتشار 2010-01-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023